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Two-photon laser scanning microscopic (TPLSM) images of FM1-43 vesicular release from Mossy fiber terminals in field CA3 of hippocampal slices confirm that release probability is reduced by chronic Pb2+ exposure.

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posted on 2015-05-26, 15:54 authored by Xiao-lei Zhang, Sara R. Guariglia, Jennifer L. McGlothan, Kirstie H. Stansfield, Patric K. Stanton, Tomás R. Guilarte

(A) Representative TPLSM pseudocolor images of FM1-43 loaded presynaptic terminals in proximal stratum radiatum of field CA3c in hippocampal slices from Pb2+-treated (Lead) and Control rats imaged prior to (0 min) and after 10 minutes (10 min) of 2Hz Mossy fiber stimulation (Calibration Bar: 5 μm). (B) Time course (Mean ± SEM) of stimulus-evoked FM1-43 destaining from large Mossy fiber puncta in field CA3 of hippocampal slices in response to 2Hz Schaffer collateral stimulation (solid bar) in control (solid circles, N = 5 slices, 25 puncta) versus chronically Pb2+-treated (open circles, N = 6 slices, 27 puncta) rats. Overall, decay time constants were not statistically significantly smaller in slices from Pb2+-treated rats (white bars) compared to those from control rats (black bars). However, a significant effect of Pb2+ was observed at the later time points in the 2Hz train (gray bar; * P < 0.05, Student’s t-test comparing open and black squares).