Two-color correlative PALM and SEM data of nuclear lamina proteins.

(A) PALM image of lamin A-PS-CFP2. (B) PALM image of lamin B1-mEos2. (C) Combined lamin A-PS-CFP2 and lamin B1-mEos2 PALM data. (D) Combined two-color lamin protein PALM data correlated with electron micrograph. (E-H) Higher magnification views from boxed area in D of PALM image of lamin A-PS-CFP2 (E), PALM image of lamin B1-mEos2 (F), combined lamin A-PS-CFP2 and lamin B1-mEos2 PALM data (G), and combined two-color lamin protein PALM data correlated with electron micrograph (H). Note the cytoplasmic organelles such as mitochondria (M). White arrows in panels A-D indicate Au beads that are used to align the datasets.