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Transgenic unc79, unc80 or na expression produces increased protein levels in wild-type and mutant backgrounds.

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posted on 2013-11-05, 03:02 authored by Bridget C. Lear, Eric J. Darrah, Benjamin T. Aldrich, Senetibeb Gebre, Robert L. Scott, Howard A. Nash, Ravi Allada

(A-C) Western blot analyses were used to label UNC79, UNC80, and NA proteins from adult head extracts; representative blots are shown. For all blots shown, lane 1  =  elavGAL4/+ (no UAS); lanes 3,5,7  =  UAS/+ (no GAL4); lanes 2,4,6,8  =  elavGAL4; UAS/+. (A) Pan-neuronal expression of UAS-unc79MYC 23-24 in wild-type (lane 2), nahar (lane 4), unc79x25 (lane 6), or unc80x42 (lane 8) backgrounds. (B) Expression of UAS-HAunc80 1M in wild-type (lane 2), nahar (lane 4), unc79x25 (lane 6), or unc80x42 (lane 8) backgrounds. (C) Expression of UAS-na U3 in wild-type (lane 2), nahar (lane 4), unc79x25 (lane 6), or unc80x42 (lane 8) backgrounds.

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