Fig_5.tif (717.38 kB)
The abnormal cell cycle phenotype of yih1Δ cells does not depend on Gcn2 or Gcn1.
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posted on 2015-07-15, 05:45 authored by Richard C. Silva, Martina Dautel, Bruno M. Di Genova, David C. Amberg, Beatriz A. Castilho, Evelyn Sattlegger(A) Representative histograms of DNA content of asynchronous cultures: wild type (H1511), and yih1Δ (ESY11001b), gcn2Δ (H2557) and gcn1Δ (H2556) single mutants, and yih1Δ;gcn2Δ (ESY10075aa) or yih1Δ;gcn1Δ (ESY10075aa) double mutants, grown to log-phase in YPD, stained with PI and analyzed by flow cytometry; the distribution of cells in G1 (1C), S or G2/M (2C) is shown. (B) The proportion of cells in G1 (1C), S or G2/M (2C), as determined from (A) is given as percentage of the total cell number, quantified using the flow cytometry gates depicted in A with the cell-cycle tool (Watson model) of Flowjo software, 9.3.3 version. Data are presented as means ± S.E. (error bars) of three independent experiments. * p< 0.05 (ANOVA).
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YIH 1 deletionGcn 2 Regulator Yih 1 InteractsYih 1Cdc 28 binding partnerYih 1 displaysCyclin Dependent Kinase Cdc 28Gcn 2 activityAmino acid substitutionsrwdPromotes Cell Cycle ProgressionBimolecular fluorescence complementationCdc 28. Yih 1helix H 2dnakinase GCN 2Gcn 1 bindingcdkCDC 28impactcell cycleYih 1 modulates
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