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Supervised clustering analysis with expression data of the 999 genes induced by CRT in a good responder cluster.

figure

posted on 2015-12-01, 15:39 authored by Yosuke Tanaka, Kazuhiko Aoyagi, Keiko Minashi, Rie Komatsuzaki, Masayuki Komatsu, Fumiko Chiwaki, Masashi Tamaoki, Takao Nishimura, Naoki Takahashi, Ichiro Oda, Yuji Tachimori, Tokuzo Arao, Kazuto Nishio, Shigehisa Kitano, Kenta Narumi, Kazunori Aoki, Satoshi Fujii, Atsushi Ochiai, Teruhiko Yoshida, Manabu Muto, Yasuhide Yamada, Hiroki Sasaki

(A) In accordance with the unsupervised clustering analysis with expression data of the processed 2,854 genes, the dendrograms reproducibly showed the presence of a good responder cluster (CR: 56% and 68%) in both pre- and post- treatment samples. (B) Comparison of immune-related gene expression levels between pre- and post- treatment samples. Key genes (PRF1, GZMB, IFNG, CASPs, TNFs) for the CTL activation, which were included in 234 immune-related genes of the above 999 genes, were upregulated in post-treatment samples especially with CR cases. Pre-treatment samples (blue), post-treatment samples (red). CR: cases with complete response, non CR: cases with non CR (partial response, PR). * p<0.05, **p<0.01, ***p<0.001.