Sumo binds non covalently the recombinant RGSZ2 protein.
A. Recombinant RGSZ2 and its RH region were incubated with SUMO1- (lane 3), SUMO2- (lane 4) and SUMO3-agarose (lane 5). SUMO-agarose conjugates captured RGSZ2 and its RH domain. Lane 1, RGSZ2/RH added. Lane 2, RGSZ2/RH in presence of agarose without SUMO. B. The Gαi2 subunit shows no binding to SUMO1/2/3-agarose.