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Sumo binds non covalently the recombinant RGSZ2 protein.

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posted on 2011-12-06, 01:47 authored by Javier Garzón, María Rodríguez-Muñoz, Ana Vicente-Sánchez, María Ángeles García-López, Ricardo Martínez-Murillo, Thierry Fischer, Pilar Sánchez-Blázquez

A. Recombinant RGSZ2 and its RH region were incubated with SUMO1- (lane 3), SUMO2- (lane 4) and SUMO3-agarose (lane 5). SUMO-agarose conjugates captured RGSZ2 and its RH domain. Lane 1, RGSZ2/RH added. Lane 2, RGSZ2/RH in presence of agarose without SUMO. B. The Gαi2 subunit shows no binding to SUMO1/2/3-agarose.


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