Fig_1.tif (2.94 MB)

Schaffer collateral-CA1 paired-pulse facilitation (PPF) is enhanced in chronically Pb2+-exposed rat hippocampus, indicative of reduce transmitter release.

Download (0 kB)
posted on 2015-05-26, 15:54 authored by Xiao-lei Zhang, Sara R. Guariglia, Jennifer L. McGlothan, Kirstie H. Stansfield, Patric K. Stanton, Tomás R. Guilarte

(A) Representative excitatory postsynaptic currents (EPSC) in field CA1 in response to Schaffer collateral paired-pulse stimuli at a 30 ms interstimulus interval (ISI) in a hippocampal slice from a control (black trace) versus a Pb2+-treated (red trace) rat. (B) Mean ± SEM ratio of P2/P1 (30ms ISI) at Schaffer collateral-CA1 synapses in slices from control (black, N = 13 slices) versus Pb2+-treated (red, N = 9 slices) rats, showing that PPF was significantly larger in Pb2+-treated rats (*, P<0.05, Student’s t-test). (C) Representative EPSCs evoked by the second of two Schaffer collateral stimuli in a CA1 pyramidal neuron, illustrating the ISI range of PPF. (D) Mean ± SEM EPSC PPF P2/P1 ratio as a function of ISI, where PPF was significantly enhanced for ISI 10–125 ms in slices from Pb2+-treated (red circles) versus control (black circles) rats (*, P<0.05, ANOVA for repeated measures and post-hoc Student’s t-test with Bonferroni correction).