Rsc1 and Rsc2 have distinct functions in mediating DNA damage hypersensitivity.

(A) and (B) Serial dilutions of rsc1 and rsc2 mutant strains harbouring the indicated expression construct were plated onto media containing the indicated dose of phleomycin. (C) Survival after 1 hour of 0.1% MMS treatment in G2/M arrested wt, rsc1, rsc2, or rad52 mutant strains relative to untreated G2/M arrested cells. (D) Survival after 1 hour of 0.1% MMS treatment in G1 arrested wt, rsc1 or rsc2 mutant strains relative to untreated G1 arrested cells. (E) Relative survival of wt, rsc1 or rsc2 mutant strains in the JKM179 strain background (which carries the HO cleavage site in the MAT locus) or the YPF17 strain background (which carries the HO cleavage site in the LEU2 gene) in the presence of continued transcriptional induction of the HO endonuclease.