Restore WASF3 expression impairs miR-217-induced inhibition of proliferation and invasion in osteosarcoma cells.
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(A) Western blot analysis of WASF3 in MG-63 cells co-transfectedwith either miR-217 mimic or scramble and 2.0 µg pCDNA-WASF3 orpCDNA empty vector. (B) The relative WASF3 protein was showed with different combinations. The signal in each lane was quantified using ImageJ software and the ratio of WASF3 to GAPDH was determined. (C) Cell growth curves in MG-63 cells transfected with different combinations using CCK-8 analysis. (D) The relative ratio of wound closure per field with different combinations is shown. (E) Transwell analysis of MG-63 cells treated with different combinations. The invasive cells per field is shown right. (F) Wound healing assays of MG-63 cells after treatment with different combinations. (G) Western blot analysis of WASF3 expression in 8 miR-217 down-regulated osteosarcoma tissues and their pair-matched nontumor tissues. GAPDH was also detected as a loading control. All data uses t test and is shown as mean ±SEM. *p<0.05,** p<0.01,***p<0.001.