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Responses of mouse macrophages and human monocytes to LPS, MPL, and dLOS.

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posted on 2014-01-22, 03:16 authored by Ji Eun Han, Seo Ri Wui, Kwang Sung Kim, Yang Je Cho, Wan Je Cho, Na Gyong Lee

(A) Mouse peritoneal macrophages were cultured with LPS, MPL, or dLOS for 24 h. TNF-α, IL-6, and IL-12 levels released into culture media were determined by sandwich ELISA. Data are expressed as mean ± standard deviation (SD) of triplicate cultures and represent two independent experiments with similar results. *, P<0.05; **, P<0.01; ***, P<0.001 as compared with MPL-treated cells. (B) Human PBMCs and monocytes were cultured with LPS, MPL, or dLOS. Culture supernatant was harvested at 12 h or 24 h and assayed for TNF or IL-12 production, respectively. Data represent three independent experiments with similar results.

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