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Representative guanidine denaturation curves of proteins.

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posted on 2016-01-18, 15:12 authored by Qiang Wang, Nicklas Waterhouse, Olusegun Feyijinmi, Matthew J. Dominguez, Lisa M. Martinez, Zoey Sharp, Rachel Service, Jameson R. Bothe, Elliott J. Stollar

AbpSH3 WT (open square), V21K (open triangle), E7L (line), Triple (open circle) and HLL (open diamond) denaturation monitored by tryptophan fluorescence emission at 330 nm. Fluorescence values are expressed as a fraction of the total change. The lines joining the points in each graph are theoretical fits to the data based on Eq 4 and the resultant thermodynamic parameters are shown in Table 2.