Quantification of MSCA-1⁺ cells under DMEM- and MC-XF culturing conditions by FACS analysis.

Unseparated JPCs were plated in culture dishes in DMEM medium. For the first test runs, cells were maintained in DMEM medium whereas the second series underwent stepwise FCS reduction and convertion to MC-XF medium. At the same time points (day 2, 5 and 9 after conversion from DMEM to MC-XF culture conditions), cells were detached from the dishes and the percentages of MSCA-1⁺ cells were determined by FACS analysis. Significant higher amounts of MSCA-1⁺ cells were detected under MC-XF culturing conditions at day 2 and 5 (p<0.01 and p<0.05).