Figure_5.tif (1.15 MB)
Protein localization results of B42.
figure
posted on 2012-05-07, 01:01 authored by Aurelie Tomczak, Jana Sontheimer, David Drechsel, Rainer Hausdorf, Marc Gentzel, Andrej Shevchenko, Stefanie Eichler, Karim Fahmy, Frank Buchholz, M. Teresa PisabarroA) Westernblot analysis of the C-terminal GFP-tagged B42 transgenic cell pool using an antibody to GFP. A weak band of the expected size 39.8 kDa (GFP-tag: 31 kDa+B42 8.8 kDa) was detected (marked with *). B) Immunoflourescent staining of the transgenic cell line. Two positive and two negative cells of the cell pool are shown. Localization of the C-terminal GFP-tagged B42 protein is mainly in the cytoplasm of HeLa cells: GFP - green, alpha-tubulin - red, DNA (DAPI) – blue. C) Only GFP-signal of B is shown in grey scale.
