Prenatal metformin exposure leads to elevated fasting glucose during HFD in the male offspring.
Fasting glucose was significantly elevated after a 10-week HFD intervention in metformin exposed male offspring. Time points: RD = 9–10 weeks, early HFD 16 weeks, late HFD = 19 weeks. Black circles (•) = prenatal control, white circles (○) = prenatal metformin (n = 12–16). Data expressed as mean ± SEM. ***P<0.0001 by Bonferroni post hoc tests.