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Potassium ion release by phage P22 infection.

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posted on 2013-08-12, 04:04 authored by Justin C. Leavitt, Lasha Gogokhia, Eddie B. Gilcrease, Anshul Bhardwaj, Gino Cingolani, Sherwood R. Casjens

A. Infection of Salmonella strain UB-0001 by P22 clear mutant phage UC-0011 at different multiplicities of infection (MOIs). Full strain genotypes at given in Table 2 of article text. B. Infection of Salmonella UB-0001 at 30° and 37°C by phage P22 UC-0011. C. Infection of Salmonella host strains that have (UB-0001) or do not have (UB-2130) P22’s O-antigen surface polysaccharide receptor by phage P22 UC-011; potassium ion release by uninfected Salmonella UB-0001 is also shown for comparison. D. Infection of a Salmonella host that has no P22 prophage (UB-0001) and a host that carries a P22 prophage that expresses its repressor (c2) gene but is missing the sieA and gtrABC genes (UB-0134) by P22 UC-0011. E. Infection of Salmonella UB-0001 by P22 phages that carry two mutations in the tail needle knob that should abrogate L-glutamate binding (see Table 2 of article text for amino acid changes). All infections were carried out at 30°C and MOI of 10 unless otherwise indicated. F. Infection of Salmonella UB-0001 by P22 UC-0011 with and without 10 mM L-glutamate added to the medium outside of the cells. Potassium ion measurements were performed as described in Materials and Methods of article text.