Figure_4.tif (307.09 kB)
PhrB contains an Fe-S cluster.
figure
posted on 2011-10-31, 02:23 authored by Inga Oberpichler, Antonio J. Pierik, Janine Wesslowski, Richard Pokorny, Ran Rosen, Michal Vugman, Fan Zhang, Olivia Neubauer, Eliora Z. Ron, Alfred Batschauer, Tilman LamparterX band EPR spectra of PhrB and PhrA (normalized to equal protein concentration). Photoreduction was for 5 min under anaerobic conditions with white light (slide projector). Incubation with sodium dithionite (2 mM) or potassium ferricyanide (2 mM) was for 5 min at 23°C. For comparison the EPR spectra of (3Fe-4S)1+ in aconitase (H2O2-treated yeast mitochondria) and of the (4Fe-4S)3+ cluster in Allochromatium vinosum HiPIP are shown (arbitrary scaling). EPR conditions: microwave power, 0.2 mW; microwave frequency 9.458 GHz; modulation frequency, 100 kHz; modulation amplitude, 1.25 mT; temperature, 15 K.
