posted on 2013-06-13, 02:37authored byKatia M. Bottos, Anselmo G. Oliveira, Patrícia A. Bersanetti, Regina F. Nogueira, Acácio A. S. Lima-Filho, José A. Cardillo, Paulo Schor, Wallace Chamon
Corneas with intact epithelium (A, B, and C) after exposure to 0.1% riboflavin-dextran solution for 30 minutes (A) and for 240 minutes (B), as well as after exposure to concentrated 1% riboflavin-dextran for 240 minutes (C). De-epithelialized cornea soaked with 0.1% riboflavin-dextran for 30 minutes - standard technique (D). The yellow staining shows that riboflavin diffused into the stroma only when the epithelium was removed.