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Phenotypic characterization of SPP-exposed human moDCs.

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posted on 2012-12-14, 01:29 authored by Kitti Pazmandi, Brahma V. Kumar, Krisztina Szabo, Istvan Boldogh, Arpad Szoor, Gyorgy Vereb, Agota Veres, Arpad Lanyi, Eva Rajnavolgyi, Attila Bacsi

Cells were treated with freshly isolated SPPs, DPI-treated SPPs, and heat-inactivated SPPs, individually and in combination with NADPH for 24 h. Expression of HLA-DQ and co-stimulatory molecules was analyzed by means of flow cytometry. Data are presented as means ± SEM of four independent experiments. * p<0.05; ** p<0.01; *** p<0.001 vs untreated DCs, # p<0.05; ## p<0.01. (DPI: diphenyleneiodonium; SPP: subpollen particle; SPPH: heat-inactivated SPP; SPPSUP: supernatant of SPPs).

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