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Phenotypic characterization of SPP-exposed human moDCs.

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posted on 14.12.2012, 01:29 by Kitti Pazmandi, Brahma V. Kumar, Krisztina Szabo, Istvan Boldogh, Arpad Szoor, Gyorgy Vereb, Agota Veres, Arpad Lanyi, Eva Rajnavolgyi, Attila Bacsi

Cells were treated with freshly isolated SPPs, DPI-treated SPPs, and heat-inactivated SPPs, individually and in combination with NADPH for 24 h. Expression of HLA-DQ and co-stimulatory molecules was analyzed by means of flow cytometry. Data are presented as means ± SEM of four independent experiments. * p<0.05; ** p<0.01; *** p<0.001 vs untreated DCs, # p<0.05; ## p<0.01. (DPI: diphenyleneiodonium; SPP: subpollen particle; SPPH: heat-inactivated SPP; SPPSUP: supernatant of SPPs).

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