Pharmacological profile and gating of ASICs in NG2 cells.

(A) Top, ASIC currents evoked by pH 6 in the control (black) and by co-application of pH 6 and 100 µM amiloride (gray). Traces in the control and in the presence of amiloride were obtained from the same cell. Bottom, summary of the effect of amiloride on ASIC currents. Data obtained from the same cell were connected by dashed lines. ***P<0.0005. (B) Top, ASIC currents evoked in the control (black) and after bath addition of PcTX1 (gray). Bottom, summary of the effect of PcTX1 on ASIC currents. **P<0.005. (C) Representative ASIC currents in a nucleated patch (V-clamped at −60 mV) evoked by double pulses (2 s, pH 5) separated by 20 s. (D) Recovery from desensitization of ASIC currents. The black curve represents a single exponential function fitted to the data points (pooled from at least 5 cells). ASIC recovery curves from BCs (red) and O-LM cells (blue) (reproduced from [24]).