Figure_3.tif (770.67 kB)
Percentage neutralization with Mabs and sCD4 performed in plaque reduction assay using GHOST(3) or U87.CD4 (CCR5- or CXCR4-expressing) cells of viruses as indicated.
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posted on 2009-02-20, 02:08 authored by Eva Maria Fenyö, Alan Heath, Stefania Dispinseri, Harvey Holmes, Paolo Lusso, Susan Zolla-Pazner, Helen Donners, Leo Heyndrickx, Jose Alcami, Vera Bongertz, Christian Jassoy, Mauro Malnati, David Montefiori, Christiane Moog, Lynn Morris, Saladin Osmanov, Victoria Polonis, Quentin Sattentau, Hanneke Schuitemaker, Ruengpung Sutthent, Terri Wrin, Gabriella ScarlattiCut-off is set at 30% neutralization (3 SD above the negative control, based on intra-assay variation). GHOST(3) cells contain GFP which is activated upon HIV infection and green cells can be counted in a fluorescence microscope 3 days after infection. The readout in HIV-infected U87.CD4 cultures is light microscopic counting of syncytial cells (single or groups of syncytia) after fixation and heamatoxylin staining.
