Participation of PLCγ1 in the feedback monomerization of the EGFR.
Figures are generally photos, graphs and static images that would be represented in traditional pdf publications.
(A) ccRICS analysis of PLCγ1 binding to EGFR upon EGF challenge. 2D cross-correlation of eGFP-EGFRwt and mCherry-PLCγ1wt in CHO-K1 cells before (left) and after (right) EGF addition. (B) Cross-correlation index (CCi) between mCherry-PLCγ1, eGFP-EGFR, and their mutants before (white) and after (grey) EGF addition. Lyn-mCherry + eGFP (1) and lyn-mCherry + lyn-eGFP (2) were used as negative controls (CTRL). Data are means ± SD. Symbol * indicates significant difference with p < 0.005 calculated using a two sided T-test with unequal variance. (C) Time traces of diffusion coefficient of eGFP-EGFRY992/1148/1173F, lacking PLCγ binding sites in the upper panel, and eGFP-EGFRwt coexpressed with mCherry-PLCγ1Y783F (inactive mutant, lower panel).