Normal survival and steady-state proliferation of SIT-deficient CD4⁺ T cells.

Lymph node cells were isolated and stained with CD4 and CD8 mAbs. A) BrdU incorporation was measured for CD4⁺ T cells. Bar graphs indicate mean values ± SEM from 3 SIT⁻/⁻ and 5 SIT⁺/⁺ mice. B) Intracellular Bcl-2 expression in CD4⁺ T cells from SIT⁻/⁻ (empty histogram) and SIT⁺/⁺ (filled histogram) mice. Isotype control is shown as a gray line. C) Annexin V expression on CD4⁺ T cells. Bar graphs indicate mean values ± SEM from 3 SIT⁻/⁻ and 3 SIT⁺/⁺ mice.