Neuromuscular morphology.

(A) Confocal images of anti-HRP immunofluorescent staining showing NMJ 6/7, segment A2 of mid-third-instar Drosophila larvae. D42>w¹¹¹⁸ or D42>khc^wt larvae were used as controls for the two mutant phenotypes D42>khc^N262S and D42>khc^wt+N262S. Larvae were raised at 29°C. Frequently, boutons with abnormal anti-HRP accumulation (A, arrowheads) are observed in Khc^N262S-expressing larvae. Scale bars: 10 µm, right panels 4 µm. (B, C) Quantification of NMJ area. Analysis was performed in both (B) the anterior segment A2 and (C) the posterior segment A5. (D, E) Quantification of synapse numbers (glutamate receptor fields) in segments A2 and A5. n = 8–10 NMJs per genotype and per segment were used for B–E. A one-way ANOVA followed by a Tukey-Kramer post-test was used for (B,C). Statistical significance in (D,E) was determined using a Kruskal-Wallis H-test followed by a Dunn's test for comparisons between multiple groups. * p<0.05; ** p<0.01. The standard error of the mean (s.e.m.) is shown as a box, the standard deviation (s.d.) as a black line.