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Morphological and phenotypical changes in T-cells during sensitization.

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posted on 2010-02-26, 00:31 authored by Ali S. Arbab, Branislava Janic, Kourosh Jafari-Khouzani, A. S. M. Iskander, Sanath Kumar, Nadimpalli R. S. Varma, Robert A. Knight, Hamid Soltanian-Zadeh, Stephen L. Brown, Joseph A. Frank

Upper panel: Culture of cord blood derived T-cells in the absence (left) or presence (right) of U251 cell lysate primed irradiated DC (PIDC). T-cells were cultured in RPMI-1640 media containing 10% FBS and IL-2 for 6 days. Note the number and morphology of the sensitized T-cells (right). Middle panel: Dot plot analysis of flow cytometric data of the CTLs showing activated T-cells (CD25) that are also both CD4 and CD8 positive. Lower panel: Flow-cytometric analysis (histogram plot) of T-cells cultured in the absence (red line) and presence (black line) of PIDC for day 6. Note the higher number of CD4+, CD8+ and CD25+ T-cells on day 6. There were a significantly (p = 0.006) higher number of activated T-cells on day 6 in the presence of PIDC.

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