Message levels of Meis1, Pbx1 and Hox family members in fetal liver cells.

A. QPCR of relative Gfi1b and Meis1 mRNA levels (normalized for HPRT) in embryonic day 12.5 (e12.5) wild type, gfi1b+/− and gfi1b−/− fetal liver cells. B. QPCR of relative Meis1 mRNA levels (normalized for HPRT) in control (wild type or gfi1b+/−) and gfi1b−/− fetal liver cells differentiated along the erythroid or megakaryocytic lineages ex-vivo. C. QPCR of relative Hoxa9, Pbx1, Hoxb3, Hoxc4 and Hoxd13 message levels from total fetal liver cells of the indicated genotypes. Averages and standard deviations from multiple embryos of different genotypes from three cohorts are shown. The p values for Pbx1, Hoxb3 and Hoxc4 levels in wild type versus mutant cells were <0.001 and those for Hoxa9 and Hoxd13 were <0.01. All values <0.05 were considered significant.