Leukocyte count and composition across induced menstrual-like bleeding in mice.

Single cell suspensions were generated from freshly isolated uteri by enzymatic digestion and used for FACS analysis. A: Representative FACS plots gated on live CD45⁺ cells are shown. B: Diagram depicts mean ± SD of frequencies of indicated cell populations among total CD45⁺ leukocytes. C-D: Graphs show total cell number of CD45⁺ leukocytes (C) or of indicated leukocyte subpopulations (D) per uterus horn (symbols  =  data from individual mouse; black line  =  mean). One out of 4 independent experiments is shown (n = 5–6 per experiment). Significance tested by one-way Anova/Tukey test (* p≤0.05, ** p≤0.01, *** p≤0.001).