L-type/R-Type Ca²⁺_o channel blockers rescue Ca²⁺_o-dependent inhibition of lung branching morphogenesis.

Effect of the calcium channel blockers, SNX-482 and nifedipine, in the presence of 1.70 mM Ca²⁺_o on lung branching morphogenesis in C57BL/6 E12.5 mouse lungs after 48 h in culture. Lungs cultured in presence of 1 µM SNX-482 and 1 µM nifedipine showed significant rescue of Ca²⁺-dependent suppression of branching compared to those cultured in medium containing 1.70 mM Ca²⁺_o alone. Data were pooled from 3 – 4 separate isolations for each condition and are presented as mean ± SEM. *, p<0.05; **, p<0.01; ***, p<0.001.