In vivo imaging of bioluminescent T.

brucei GVR35 over the course of infection. GVR35 WT or -LUC2-infected mice were imaged at different times after infection. Total flux in photons per second (p/sec) for the mouse body (ventral view) or head region (dorsal view) is shown. D-luciferin (150 mg/kg) was injected intraperitoneally 10 minutes before imaging. ND indicates that trypanosomes were not detected in blood samples. Images show the same two representative mice over the full course of infection. Images of a GVR35 WT-infected mouse are shown to indicate the background bioluminescence. The colour scale indicates bioluminescent radiance in photons.second⁻¹.centimeter⁻².steradian⁻¹.