Immunostaining of ECM proteins in BM.

(A) Immunofluorescence for nidogen 1 (NID1) and nidogen 2 (NID2) shown in original 40× and 100× magnification (Scale Bar: 100 µm). There is no difference in staining intensity observed between WT and transgenic mice (1mice/group, 3 independent experiments). Image is representative. Green∶nidogen 1 and nidogen 2, respectively. BM: Bruch's membrane; ILM: inner limiting membrane; RPE: retinal pigment epithelium. Image of H&E stained sections in 40× and 100× magnification provides orientation.