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IQGAP1 activates β-catenin transcription and promotes its translocation to nucleus.

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posted on 07.08.2015 by Xuewen Jin, Yuling Liu, Jingjing Liu, Weiliang Lu, Ziwei Liang, Dan Zhang, Gang Liu, Hongxia Zhu, Ningzhi Xu, Shufang Liang

(A) IQGAP1 overexpression by transfecting with pFlag-IQGAP1 plasmids (flag-IQ) upregulated β-catenin mRNA level. Transfection with empty pCMV6 plasmids was taken as a control. And the mRNA level of c-myc, cyclin D1 and Axin2 upon IQGAP1 overexpression was shown in (D). (B) IQGAP1 knockdown, by specific siRNA for IQGAP1 (si-IQ), decreased β-catenin mRNA level. Transfection with non-target siRNA was taken as a control (si NC). (C) IQGAP1 activates β-catenin transcription by a luciferase promoter analysis. (E) The overexpression of IQGAP1 induced β-catenin translocation from cytoplasm to nucleus. LMNB1 was a nuclear protein control. GAPDH was a cytoplasmic protein control. ** Student’s t test p<0.01, *** Student’s t test p<0.001.