Histological appearance of chronic soft-tissue infection.

A, C) Mice received CLIO at day 2 post infection. Images were recorded 24 h after administration. The muscle was recovered immediately after imaging. B) Mice received PFC at day 2 post infection and were imaged 24 h after administration. The muscle was recovered immediately after imaging. A) Macrophages and Granulocytes are embedded in granulation tissue at the abscess rim of CLIO group mice. C) Iron-specific staining demonstrates iron deposits in granulocytes and macrophages (iron appears blue). B) HE-staining of PFC group mice reveals macrophages and granulocytes in fibroblast-enriched granulation tissue similar to CLIO group mice.