Figure_5.tif (978.01 kB)
Hc-CRA AADM mutants lack the splice acceptor site of intron 14.
figure
posted on 2009-04-10, 00:34 authored by Lucien Rufener, Pascal Mäser, Isabel Roditi, Ronald KaminskySequencing of PCR products amplified from genomic DNA revealed a 10 bp deletion in the Hc-CRA AADM mutant that encompasses the predicted splice acceptor site (bold). The blue box corresponds to the end of intron 14 and the yellow box to the start of exon 15. Asterisks denote bases identical throughout all 12 sequenced clones.
