HSPB4 prevents the aggregation of HSPB5 mutants in C2C12 myoblasts.

Mouse myoblast C2C12 cells were co-transfected with myc-HSPB5 mutants (R120G, 450 Δ A, 464 ΔCT) and FRTTO as a control (1:0 ratio) or HSPB4-V5 (at increasing ratios 1:1, 1:2, 1:3). 48 h after transfection, cells were lysed in Triton X-100 and fractionated into Soluble (S) and Pellet (P) and western blot was performed. The percentage of insoluble HSPB5 mutants in each case was quantified ([p]_density*100 / ([p] _density + [s] _density)) and is depicted in the chart above the blot.