HA-specific CD8⁺ T cells from VILLIN-HA/CL4-TCR exhibit suppressive capacity.

HA-specific CD8⁺ T cells were isolated from the MLN of CL4-TCR and VILLIN-HA/CL4-TCR transgenic mice by cell sorting and co-cultured with CFSE-labeled HA-specific CD4⁺ or CD8⁺ responder cells in the presence of the cognate peptide. Proliferation of responder cells was measured by loss of CFSE dye. Data shown are representative of three independent experiments.