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Growth phenotype of cp-pgm plants.

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posted on 2014-11-17, 03:34 authored by Irina Malinova, Hans-Henning Kunz, Saleh Alseekh, Karoline Herbst, Alisdair R. Fernie, Markus Gierth, Joerg Fettke

A, Seeds were sowed on MS medium containing sucrose and antibiotics (kanamycin [50 µg/mL], hygromycin [50 µg/mL]). Plants were grown under long day conditions (16 h light/8 h dark) and were two-week-old. Bar = 1 cm. B, cp-pgm plant before trypan blue staining. C, Col-0 and cp-pgm plants after trypan blue staining. The cp-pgm plant was five- week-old, germinated on MS plate (as above) and the two last weeks grown under continuous illumination. Leave of Col-0 from three-week-old plant grown under 12 h light/12 h dark conditions. Bars = 1 cm. D–F, Phenotype of cp-pgm plants under continuous illumination. Seeds were germinated on MS medium containing sucrose with antibiotics (kanamycin [50 µg/mL], hygromycin [50 µg/mL]). After four weeks plants were transferred to soil and grown further under continuous illumination. D, Plant was six-week-old. Bar = 1 cm. E–F, Flower buds of cp-pgm transgenic plants. Plant was six-week-old (E) and seven-week-old (F). Bars = 1 mm.

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