Galectin-4 inhibits activation, cell cycle progression, and expansion of activated T cells.

(A) PBMC were stimulated with anti-CD3 and cultured for 72 h in the presence or absence of 100 µg/ml Gal-4. CD80 and CD86 positive cells were detected by flow cytometric analysis. Data represent mean±SEM of three individual experiments. *p≤0.05 for decrease vs. baseline. (B) Flow cytometric analysis of cyclin-A expression of anti-CD3/CD28 stimulated T cells cultured in the presence or absence of 100 µg/ml Gal-4. Data are representative for four individual experiments. (C) Flow cytometric analysis of cell cycle progression and cyclin-B1 expression of anti-CD3/CD28 stimulated T cells cultured in the presence or absence of 100 µg/ml Gal-4. Data are representative for four individual experiments. (D) PBT were transfected with Gal-4 siRNA or scrambled control. After stimulation with IL-2 cell cycle progression and cyclin B1 expression were determined. Data are representative of three individual experiments. (E) Anti-CD3/CD28 activated PBT were stained with CFDA and T cell expansion was determined after four days of incubation in the presence or absence of 100 µg/ml Gal-4 by flow cytometric analysis. Data are representative of three individual experiments.