Foxa2 regulates the expression of FTO in HEK 293 cells.

HEK 293 cells were transiently transfected with either a control vector (pHD vector) or a vector containing full coding sequence of Foxa2 (pHD-Foxa2). In the RNA interference group, cells were transfected with siRNA against Foxa2 (Foxa2 siRNA) or negative control siRNA (NTC). After 48 h, cells were harvested and FTO expression was determined by qPCR and Western analyses. Bar graph depicts the relative mRNA and protein expression levels of FTO and Foxa2 as quantified by the Image Lab software (BIO-RAD) and normalized to GAPDH. Values represent the mean±SE of 3 independent experiments. (*P<0.05, **P<0.01).