Figure 7. The HRS3-scFv-IL12-Fc-IL2 dual cytokine fusion protein bound to CD30⁺ cells activates resting NK cells to IFN-γ secretion.

CD30⁺ and CD30⁻ MC38 cells (5×10⁴ cells/well) were incubated with equimolar amounts of anti-CD30 fusion proteins for 1 h on ice. Unbound fusion proteins were either present during the assay or washed out. Freshly isolated NK cells (5×10⁴ cells) were co-incubated for 48 hrs. IL2 (500 U/ml) was added for comparison. NK cell activation was recorded by monitoring IFN-γ secreted into the culture medium. Data represent the mean of triplicates ± standard error of mean (SEM). An experiment out of two with similar results is shown.