Figure 2. Western blot analysis of the anti-CD30 antibody-cytokine fusion proteins.

(A) Supernatants containing HRS3-scFv-IL12 and HRS3-scFv-Fc-IL12 or HRS3-scFv-IL2 and HRS3-scFv-Fc-IL2 fusion proteins, respectively, were separated by SDS-PAGE under reducing (R) and non-reducing (NR) conditions. (B) Supernatants of 293T producer cell lines containing HRS3-scFv-Fc-IL2 and dual cytokine HRS3-scFv-IL12-Fc-IL2 fusion proteins, respectively, were separated by blue native polyacrylamide gel electrophoresis (BN-PAGE). Separated proteins were transferred to PVDF membranes and detected by staining the blots with anti-IL12 and anti-IL2 antibodies, respectively.