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FHV RNA1 replication in selected yeast deletion mutants.

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posted on 21.04.2014 by Linhui Hao, Brett Lindenbach, Xiaofeng Wang, Billy Dye, David Kushner, Qiuling He, Michael Newton, Paul Ahlquist

Yeast strains with the indicated gene deletions were transformed with plasmid pF1 (Figure 1A) and, following galactose-induction of pF1 transcription, FHV genomic RNA1 replication and subgenomic RNA3 production were assayed by Northern blot hybridization. The histograms show the averages and standard deviations of RNA1 and RNA3 levels across four independent experiments. Representative Northern blots are shown above each histogram. A). FHV wild type RNA1 replication in all 8 deletion mutants whose deleted gene functions facilitate FHV replication. WT denotes the no deletion control. B). FHV wild type RNA1 replication in a selected subsets of yeast deletion mutants whose deleted gene functions normally inhibit FHV replication. Accordingly, these strains show increased FHV replication.

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