Figure_6.tif (2.86 MB)

FCIP Responses to Direct and to Synaptic Stimulation in Acute Brain Slices

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posted on 2013-02-22, 09:16 authored by Mazahir T Hasan, Rainer W Friedrich, Thomas Euler, Matthew E Larkum, Günter Giese, Matthias Both, Jens Duebel, Jack Waters, Hermann Bujard, Oliver Griesbeck, Roger Y Tsien, Takeharu Nagai, Atsushi Miyawaki, Winfried Denk

(A) Whole-field imaged responses of Cg2-positive cells in cortex to bursts of APs evoked by somatic current injection (whole-cell recording electrode indicated schematically); responses in the recorded (red) and in a nonrecorded (green) soma and in a region with no cell body (blue).

(B) Two-photon line scan (lower trace) through the soma of a hippocampal CA1 pyramidal neuron during a burst of APs evoked by somatic current injection through a high-resistance microelectrode.

(C) Whole-field-imaged responses to synaptic stimulation in cortex (five pulses at 100 Hz, 10 μA); ΔF/F image is shown below. Fluorescence and voltage responses with and without pharmacological block of glutamate channels (note suppression of APs and unmasking of inhibitory synaptic potentials).