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Expression of luciferase in EL4 cells in the absence or presence of FasL UTR containing mmu-let-7e binding site post-vehicle or TCDD treatment.

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posted on 2013-02-19, 23:21 authored by Narendra P. Singh, Udai P. Singh, Hongbing Guan, Prakash Nagarkatti, Mitzi Nagarkatti

A: Determination of transfection efficiency of EL4 cells. EL4 cells were co-transfected with GFP containing vector and pmiRGLO-FasL plasmid and were analyzed using flow cytometry. There was more than 74% transfection of EL4 cells. B: Real-Time PCR was performed to determine luciferase expression by performing luciferase assays of EL4 cells not transfected or transfected with pmiRGLO-FasL or pmiRGLO-FasL S and exposed to vehicle or TCDD. Luciferase expression data are presented as fold change in expression. Data are depicted as mean ± SEM of three independent experiments. Asterisks (* and #) in panel B indicate statistically significant (p<0.05) difference between groups compared.