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Evidence at the protein level for nesprin-1-beta-1 in spleen and for nesprin-1-alpha-2 in cardiac and skeletal muscle.

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posted on 09.04.2014, 03:41 by Nguyen Thuy Duong, Glenn E. Morris, Le Thanh Lam, Qiuping Zhang, Caroline A. Sewry, Catherine M. Shanahan, Ian Holt

Western blot for nesprin-1 in tissues using antibody against the C-terminal region of nesprin-1 (MANNES1E 8C3). A band the size of nesprin-1-beta-1 (383 kD) was detected in spleen and bands of nesprin-1-alpha-2 (111 kD) were detected in skeletal muscle and heart.