Elimination of Wolbachia rendered TK and WX populations fully compatible.

WX females were crossed with an equal number of tetracycline-treated TK males (TK_tet♂), untreated TK males (TK♂) or WX males (WX♂). TK females were crossed with an equal number of tetracycline-treated WX males (WX_tet♂), untreated WX males (WX♂) or TK males (TK♂). Hatching rates were calculated for these crosses. Error bars represent standard errors; ns, non-significant P-value; *** P<0.0001 by t-test.