Egg preparation and fenestration.

(A) Incubator containing eggs. (B) The top of each egg is marked to indicate the location of the embryo (blastoderm). (C) Tools required for fenestration of the eggs are depicted: (from left to right) paper towels, tungsten needle and forceps, 2 egg holding devices, syringe with needle, 80% ethanol, plastic petri dish for egg shell waste, egg piercer, hacksaw, adhesive tape, scissors. (D) A pre-determined breaking point is generated after removal of 2 ml albumen to lower the level of the blastoderm. (E) After removal of the shell, the embryo is discernible on the blastoderm (arrow). (F) The egg is sealed with adhesive tape and re-incubated. (G) Capillaries are pulled before transplantation. (H) Working place (stereo-microscope with epi-illumination, diluted black ink, PBS, pipette) and (I) tools (mouth pipette, forceps, tungsten needle) required for transplantation of the cells.