Effects of epigenetic modulatory drugs on fetal alcohol induced changes in pituitary D2R mRNA levels, protein levels, pituitary weight and plasma PRL levels.
figureposted on 2015-10-28, 03:15 authored by Omkaram Gangisetty, Olivia Wynne, Shaima Jabbar, Cara Nasello, Dipak K. Sarkar
AD, PF and AF rat offspring were treated with DNA methyl transferase inhibitor 5-Aza deoxycytidine (5-AZAdC), HDACC inhibitor Trichostatin-A (TSA) alone or together during postnatal day 2–6 and after 60 days, these rats were ovariectomized and estrogen treated for 60 days. Mean ± SEM values of pituitary D2R mRNA levels (A), pituitary weight (B) and plasma PRL levels (C) are shown in the histograms. Data are mean ± SEM of n = 6 animals per group and were analyzed using one-way ANOVA with the Newman-Keuls post hoc test. *, P<0.05, between AF and controls (AD or PF). @, P<0.05 between the treatment and control in AF group.
rat chow dietalcohol exposure programsIncreases Pituitary WeightProlactinomasusceptibilityD 2R promoter methylationbinge alcohol drinkingplasma PRL levelsFetal alcohol exposureD 2R gene promoterhdacanimal modeldnmtalcohol exposure increasesdopamine D 2 receptordopamine receptor D 2D 2R mRNAalcohol exposureday 21. Control ratsDNA methylating genes