Effects of SGLT2i treatment on AGE/RAGE signaling in diabetic rats.

Quantification of AGE-positive proteins by dot blot analysis (A) and RAGE expression was assessed by Western blotting analysis with specific antibodies (B) and quantitative RT-PCR analysis (C). Representative blots are shown at the bottom of the densitometric quantifications. Serum methylglyoxal levels were assessed by HPLC-based quantification (D). Representative chromatograms are shown in supplemental Figure S7 in File S1. The data are expressed as % of control and are the means ±SEM from 7 (A), 6–7 (B) and 8–11 (C,D) animals/group. *, p<0.05 vs. control and ^#, p<0.05 vs. STZ–injected.