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Effects of E. coli supernatants on growth of Salmonella Typhimurium.

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posted on 2013-02-20, 20:26 authored by Peter Schierack, Sylvia Kleta, Karsten Tedin, Julius Tachu Babila, Sibylle Oswald, Tobias A. Oelschlaeger, Rico Hiemann, Susanne Paetzold, Lothar H. Wieler

Confluent monolayers of IPEC-J2 cells were pre- and/or co-incubated with E. coli supernatants (SN). Cells were infected with Salmonella Typhimurium using an MOI of 100∶1 Salmonella to host cells for one hour. Thereafter, numbers of extracellular, non-adherent Salmonella were determined. Growth rates are expressed as growth in percent (%) relative to Salmonella growth in cell culture medium (Salmonella mono-infection). The data are the mean ± S.E.M. of at least three separate experiments in duplicate wells. * = p<0.01 compared to Salmonella mono-infection. EcN: E. coli Nissle 1917.

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