Figure_5.tif (563.23 kB)

Effect of exposure to pneumococcus on IL-17A expression from human tissues and cells.

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posted on 2013-02-21, 09:29 authored by Ying-Jie Lu, Jane Gross, Debby Bogaert, Adam Finn, Linda Bagrade, Qibo Zhang, Jay K. Kolls, Amit Srivastava, Anna Lundgren, Sophie Forte, Claudette M. Thompson, Kathleen F. Harney, Porter W. Anderson, Marc Lipsitch, Richard Malley

A. Expression of IL-17A from tonsillar mononuclear cells from children. Tonsillar cells (n = 8) were cultured as described and stimulated with WCA or WCA derived from an isogenic, pneumolysin-negative strain (WCAply-). Stimulation with WCA was associated with significantly increased IL-17A expression compared to exposure to medium alone (P = 0.008 by Wilcoxon signed rank test), whereas stimulation with WCAply- did not increase IL-17A production. B. Expression of IL-17A from peripheral blood of adults and umbilical cord blood. Peripheral blood samples from adults (healthy adult volunteers (n = 7), parturient women (n = 11) and umbilical cord blood (n = 11) were stimulated with WCA for 6 days after which IL-17A concentration was assayed by ELISA. IL-17A production was significantly greater in adults than cord blood (P<0.001 by Mann-Whitney U test).