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Effect of a non-membrane permeable inhibitor of calmodulin on TRPV1-mediated Ca2+-transport.

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posted on 2013-02-21, 12:23 authored by Zoltán Oláh, Katalin Jósvay, László Pecze, Tamás Letoha, Norbert Babai, Dénes Budai, Ferenc Ötvös, Sándor Szalma, Csaba Vizler

Camstatin, a recently identified, selective, 25-mer polypeptide blocker of calmodulin, was employed, which inhibited capsaicin-induced Ca2+-uptake with comparable activity (IC50 = 20 µM) determined previously to other, more conventional antagonists, of calmodulin in TRPV1-NIH3T3 cells. Results with camstatin suggests calmodulin-like structure at the extracellular domains of TRPV1. Experiments were repeated two additional times in triplicate with similar results.

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